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Affinity Biosciences rabbit polyclonal anti cd34 antibody
Exosomal miR-144-3p involved in PPT regulation and MCD in response to neurogenic inflammation and peripheral nociceptor hypersensitivity. ( A ) Immunohistochemical staining images showing colocalization of <t>CD34</t> (MCs; red) and PGP9.5 (axons; green). Cell nuclei were stained with DAPI (blue). ( B ) Quantitative analyses of the colocalization of CD34 (MCs) and PGP9.5 (axons) (n = 3 per group). ( C ) The ratio of the number of cells expressing both CD34 (MCs) and PGP9.5 (axons) to the number of CD34 + MCs (n = 3 per group). All data are shown as the mean ± SD, ∗∗ P < 0.01, ## P < 0.01.
Rabbit Polyclonal Anti Cd34 Antibody, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Plasma-derived exosomal miR-144-3p targeting IL-1β is involved in pressure pain threshold regulation at PC6 acupoint in myocardial ischemia rats"

Article Title: Plasma-derived exosomal miR-144-3p targeting IL-1β is involved in pressure pain threshold regulation at PC6 acupoint in myocardial ischemia rats

Journal: Journal of Traditional and Complementary Medicine

doi: 10.1016/j.jtcme.2025.02.001

Exosomal miR-144-3p involved in PPT regulation and MCD in response to neurogenic inflammation and peripheral nociceptor hypersensitivity. ( A ) Immunohistochemical staining images showing colocalization of CD34 (MCs; red) and PGP9.5 (axons; green). Cell nuclei were stained with DAPI (blue). ( B ) Quantitative analyses of the colocalization of CD34 (MCs) and PGP9.5 (axons) (n = 3 per group). ( C ) The ratio of the number of cells expressing both CD34 (MCs) and PGP9.5 (axons) to the number of CD34 + MCs (n = 3 per group). All data are shown as the mean ± SD, ∗∗ P < 0.01, ## P < 0.01.
Figure Legend Snippet: Exosomal miR-144-3p involved in PPT regulation and MCD in response to neurogenic inflammation and peripheral nociceptor hypersensitivity. ( A ) Immunohistochemical staining images showing colocalization of CD34 (MCs; red) and PGP9.5 (axons; green). Cell nuclei were stained with DAPI (blue). ( B ) Quantitative analyses of the colocalization of CD34 (MCs) and PGP9.5 (axons) (n = 3 per group). ( C ) The ratio of the number of cells expressing both CD34 (MCs) and PGP9.5 (axons) to the number of CD34 + MCs (n = 3 per group). All data are shown as the mean ± SD, ∗∗ P < 0.01, ## P < 0.01.

Techniques Used: Immunohistochemical staining, Staining, Expressing

Related Articles

Incubation:

Article Title: Plasma-derived exosomal miR-144-3p targeting IL-1β is involved in pressure pain threshold regulation at PC6 acupoint in myocardial ischemia rats
Article Snippet: For staining, citric acid antigen retrieval solution (Solarbio, Beijing, China) was used for antigen retrieval. .. The sections were incubated with primary antibodies including a rabbit polyclonal anti-CD34 antibody (Affinity Biosciences, Cincinnati, Ohio, USA) and rabbit polyclonal anti-Protein gene product 9.5 (PGP9.5) antibody (Affinity Biosciences, Cincinnati, Ohio, USA). .. Finally, DAPI (Solarbio, Beijing, China) was used to stain the nucleus, and the cells were washed with PBS and sealed with the autofluorescence quencher Sudan Black B (Sangon Biotech, Shanghai, China) and anti-fluorescence quenching sealing tablets (Southern Biotech, Alabama, America).



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Exosomal miR-144-3p involved in PPT regulation and MCD in response to neurogenic inflammation and peripheral nociceptor hypersensitivity. ( A ) Immunohistochemical staining images showing colocalization of <t>CD34</t> (MCs; red) and PGP9.5 (axons; green). Cell nuclei were stained with DAPI (blue). ( B ) Quantitative analyses of the colocalization of CD34 (MCs) and PGP9.5 (axons) (n = 3 per group). ( C ) The ratio of the number of cells expressing both CD34 (MCs) and PGP9.5 (axons) to the number of CD34 + MCs (n = 3 per group). All data are shown as the mean ± SD, ∗∗ P < 0.01, ## P < 0.01.
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Immunofluorescence and immunohistochemical analysis in fistula tissues. (A) Representative immunofluorescence staining of <t>CD34</t> (red) and DAPI (blue) in fistula tissues from Control, PRP, and Cryo-PRP groups. (B) Quantitative analysis of CD34 expression using integrated optical density (IOD/Area). (C) Representative images of immunohistochemical staining for α-SMA, CD31, VEGF, PCNA, and TNF-α in the Control, PRP, and Cryo-PRP groups are shown (original magnification: ×400). Expression of α-SMA, CD31, VEGF, and PCNA was markedly increased in the PRP and Cryo-PRP groups compared to Control, with the highest levels observed in the Cryo-PRP group. In contrast, TNF-α expression was significantly reduced following PRP and Cryo-PRP treatment, with minimal staining in the Cryo-PRP group. (D) Quantitative analysis of immunohistochemical staining using integrated optical density per area (IOD/Area × 10 6 ). Data are presented as mean ± SD. * P < 0.05 vs. Control; *** P < 0.001 vs. Control.
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Immunofluorescence and immunohistochemical analysis in fistula tissues. (A) Representative immunofluorescence staining of <t>CD34</t> (red) and DAPI (blue) in fistula tissues from Control, PRP, and Cryo-PRP groups. (B) Quantitative analysis of CD34 expression using integrated optical density (IOD/Area). (C) Representative images of immunohistochemical staining for α-SMA, CD31, VEGF, PCNA, and TNF-α in the Control, PRP, and Cryo-PRP groups are shown (original magnification: ×400). Expression of α-SMA, CD31, VEGF, and PCNA was markedly increased in the PRP and Cryo-PRP groups compared to Control, with the highest levels observed in the Cryo-PRP group. In contrast, TNF-α expression was significantly reduced following PRP and Cryo-PRP treatment, with minimal staining in the Cryo-PRP group. (D) Quantitative analysis of immunohistochemical staining using integrated optical density per area (IOD/Area × 10 6 ). Data are presented as mean ± SD. * P < 0.05 vs. Control; *** P < 0.001 vs. Control.
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Immunofluorescence and immunohistochemical analysis in fistula tissues. (A) Representative immunofluorescence staining of <t>CD34</t> (red) and DAPI (blue) in fistula tissues from Control, PRP, and Cryo-PRP groups. (B) Quantitative analysis of CD34 expression using integrated optical density (IOD/Area). (C) Representative images of immunohistochemical staining for α-SMA, CD31, VEGF, PCNA, and TNF-α in the Control, PRP, and Cryo-PRP groups are shown (original magnification: ×400). Expression of α-SMA, CD31, VEGF, and PCNA was markedly increased in the PRP and Cryo-PRP groups compared to Control, with the highest levels observed in the Cryo-PRP group. In contrast, TNF-α expression was significantly reduced following PRP and Cryo-PRP treatment, with minimal staining in the Cryo-PRP group. (D) Quantitative analysis of immunohistochemical staining using integrated optical density per area (IOD/Area × 10 6 ). Data are presented as mean ± SD. * P < 0.05 vs. Control; *** P < 0.001 vs. Control.
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Immunofluorescence and immunohistochemical analysis in fistula tissues. (A) Representative immunofluorescence staining of <t>CD34</t> (red) and DAPI (blue) in fistula tissues from Control, PRP, and Cryo-PRP groups. (B) Quantitative analysis of CD34 expression using integrated optical density (IOD/Area). (C) Representative images of immunohistochemical staining for α-SMA, CD31, VEGF, PCNA, and TNF-α in the Control, PRP, and Cryo-PRP groups are shown (original magnification: ×400). Expression of α-SMA, CD31, VEGF, and PCNA was markedly increased in the PRP and Cryo-PRP groups compared to Control, with the highest levels observed in the Cryo-PRP group. In contrast, TNF-α expression was significantly reduced following PRP and Cryo-PRP treatment, with minimal staining in the Cryo-PRP group. (D) Quantitative analysis of immunohistochemical staining using integrated optical density per area (IOD/Area × 10 6 ). Data are presented as mean ± SD. * P < 0.05 vs. Control; *** P < 0.001 vs. Control.
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Immunofluorescence and immunohistochemical analysis in fistula tissues. (A) Representative immunofluorescence staining of <t>CD34</t> (red) and DAPI (blue) in fistula tissues from Control, PRP, and Cryo-PRP groups. (B) Quantitative analysis of CD34 expression using integrated optical density (IOD/Area). (C) Representative images of immunohistochemical staining for α-SMA, CD31, VEGF, PCNA, and TNF-α in the Control, PRP, and Cryo-PRP groups are shown (original magnification: ×400). Expression of α-SMA, CD31, VEGF, and PCNA was markedly increased in the PRP and Cryo-PRP groups compared to Control, with the highest levels observed in the Cryo-PRP group. In contrast, TNF-α expression was significantly reduced following PRP and Cryo-PRP treatment, with minimal staining in the Cryo-PRP group. (D) Quantitative analysis of immunohistochemical staining using integrated optical density per area (IOD/Area × 10 6 ). Data are presented as mean ± SD. * P < 0.05 vs. Control; *** P < 0.001 vs. Control.
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Immunofluorescence and immunohistochemical analysis in fistula tissues. (A) Representative immunofluorescence staining of <t>CD34</t> (red) and DAPI (blue) in fistula tissues from Control, PRP, and Cryo-PRP groups. (B) Quantitative analysis of CD34 expression using integrated optical density (IOD/Area). (C) Representative images of immunohistochemical staining for α-SMA, CD31, VEGF, PCNA, and TNF-α in the Control, PRP, and Cryo-PRP groups are shown (original magnification: ×400). Expression of α-SMA, CD31, VEGF, and PCNA was markedly increased in the PRP and Cryo-PRP groups compared to Control, with the highest levels observed in the Cryo-PRP group. In contrast, TNF-α expression was significantly reduced following PRP and Cryo-PRP treatment, with minimal staining in the Cryo-PRP group. (D) Quantitative analysis of immunohistochemical staining using integrated optical density per area (IOD/Area × 10 6 ). Data are presented as mean ± SD. * P < 0.05 vs. Control; *** P < 0.001 vs. Control.
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Image Search Results


Exosomal miR-144-3p involved in PPT regulation and MCD in response to neurogenic inflammation and peripheral nociceptor hypersensitivity. ( A ) Immunohistochemical staining images showing colocalization of CD34 (MCs; red) and PGP9.5 (axons; green). Cell nuclei were stained with DAPI (blue). ( B ) Quantitative analyses of the colocalization of CD34 (MCs) and PGP9.5 (axons) (n = 3 per group). ( C ) The ratio of the number of cells expressing both CD34 (MCs) and PGP9.5 (axons) to the number of CD34 + MCs (n = 3 per group). All data are shown as the mean ± SD, ∗∗ P < 0.01, ## P < 0.01.

Journal: Journal of Traditional and Complementary Medicine

Article Title: Plasma-derived exosomal miR-144-3p targeting IL-1β is involved in pressure pain threshold regulation at PC6 acupoint in myocardial ischemia rats

doi: 10.1016/j.jtcme.2025.02.001

Figure Lengend Snippet: Exosomal miR-144-3p involved in PPT regulation and MCD in response to neurogenic inflammation and peripheral nociceptor hypersensitivity. ( A ) Immunohistochemical staining images showing colocalization of CD34 (MCs; red) and PGP9.5 (axons; green). Cell nuclei were stained with DAPI (blue). ( B ) Quantitative analyses of the colocalization of CD34 (MCs) and PGP9.5 (axons) (n = 3 per group). ( C ) The ratio of the number of cells expressing both CD34 (MCs) and PGP9.5 (axons) to the number of CD34 + MCs (n = 3 per group). All data are shown as the mean ± SD, ∗∗ P < 0.01, ## P < 0.01.

Article Snippet: The sections were incubated with primary antibodies including a rabbit polyclonal anti-CD34 antibody (Affinity Biosciences, Cincinnati, Ohio, USA) and rabbit polyclonal anti-Protein gene product 9.5 (PGP9.5) antibody (Affinity Biosciences, Cincinnati, Ohio, USA).

Techniques: Immunohistochemical staining, Staining, Expressing

Immunofluorescence and immunohistochemical analysis in fistula tissues. (A) Representative immunofluorescence staining of CD34 (red) and DAPI (blue) in fistula tissues from Control, PRP, and Cryo-PRP groups. (B) Quantitative analysis of CD34 expression using integrated optical density (IOD/Area). (C) Representative images of immunohistochemical staining for α-SMA, CD31, VEGF, PCNA, and TNF-α in the Control, PRP, and Cryo-PRP groups are shown (original magnification: ×400). Expression of α-SMA, CD31, VEGF, and PCNA was markedly increased in the PRP and Cryo-PRP groups compared to Control, with the highest levels observed in the Cryo-PRP group. In contrast, TNF-α expression was significantly reduced following PRP and Cryo-PRP treatment, with minimal staining in the Cryo-PRP group. (D) Quantitative analysis of immunohistochemical staining using integrated optical density per area (IOD/Area × 10 6 ). Data are presented as mean ± SD. * P < 0.05 vs. Control; *** P < 0.001 vs. Control.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: A novel cryoprecipitate-enriched PRP (Cryo-PRP) gel with enhanced mechanical strength and regenerative capacity accelerates enterocutaneous fistula healing

doi: 10.3389/fbioe.2025.1668608

Figure Lengend Snippet: Immunofluorescence and immunohistochemical analysis in fistula tissues. (A) Representative immunofluorescence staining of CD34 (red) and DAPI (blue) in fistula tissues from Control, PRP, and Cryo-PRP groups. (B) Quantitative analysis of CD34 expression using integrated optical density (IOD/Area). (C) Representative images of immunohistochemical staining for α-SMA, CD31, VEGF, PCNA, and TNF-α in the Control, PRP, and Cryo-PRP groups are shown (original magnification: ×400). Expression of α-SMA, CD31, VEGF, and PCNA was markedly increased in the PRP and Cryo-PRP groups compared to Control, with the highest levels observed in the Cryo-PRP group. In contrast, TNF-α expression was significantly reduced following PRP and Cryo-PRP treatment, with minimal staining in the Cryo-PRP group. (D) Quantitative analysis of immunohistochemical staining using integrated optical density per area (IOD/Area × 10 6 ). Data are presented as mean ± SD. * P < 0.05 vs. Control; *** P < 0.001 vs. Control.

Article Snippet: Subsequently, the sections were incubated overnight at 4 °C with rabbit anti-CD34 antibody (BIOSS, bs-064R; 1:100 dilution).

Techniques: Immunofluorescence, Immunohistochemical staining, Staining, Control, Expressing